1Center for Clinical Studies, Webster, TX, USA
2Meharry Medical College, Nashville, TN, USA
3Department of Dermatology, University of Texas Health Science Center, Houston, TX, USA
Conflict of interest:
Stephen Tyring received grant support, paid to the Center for Clinical Studies, from Athenex. The other authors have no conflicts of interest.
Actinic keratosis (AK) is a common precancerous condition found on chronically sun-damaged skin, particularly on the face, scalp, arms, and legs. Early and effective treatment of AKs is important to prevent progression to squamous cell carcinoma. Many topical treatments for AKs are often limited because of poor tolerability, prolonged treatment duration, and reduced adherence. Tirbanibulin 1% ointment, a new topical field therapy for AKs, reduces these issues. It requires a consecutive 5-day application period and is effective, demonstrating complete (100%) clearance of AK lesions in 49% of patients, partial (>75%) clearance in 72%, and a median reduction in lesion count of 87.5% while exhibiting a favorable safety profile, mild adverse events, improved tolerability, and long-term results.
tirbanibulin; actinic keratosis, field therapy, 5-fluorouracil, diclofenac, imiquimod, face, scalp
Actinic keratosis (AK) is a common, recurrent precancerous condition found on chronically sun-damaged skin, particularly on the face, scalp, arms, and legs.1 Clinically AKs appear as macules, papules, or hyperkeratotic plaques with an erythematous background.2 Its prevalence steadily increases with age and prolonged sun-exposure. The American Academy of Dermatology (AAD) notes that approximately 60% of predisposed individuals over the age of 40 are diagnosed with at least one AK.3 Other risk factors include male gender, fair skin (Fitzpatrick type I-II), ultraviolet (UV) exposure, immunosuppression, previous history of AKs or skin cancer, human papillomavirus (HPV) infection, and genetic diseases.3
Cumulative UV exposure is considered a major risk factor for AK development because of the resultant modification of cellular repair mechanisms in keratinocytes.4 UVB irradiation causes the formation of thymidine dimers in DNA and mutations of the telomerase gene, whereas UVA indirectly induces DNA mutation through photo-oxidative stress.3 The clinical significance of AKs is secondary to the associated discomfort, cosmetic burden, and the possibility of progression to invasive squamous cell carcinoma (SCC).5 Rates of malignant transformation vary from 0.025% to 16%, and the risk of progression increases in patients with multiple AKs (more than five).3 While AKs are a risk factor for the development of SCC, it is impossible to predict which lesions will transform, therefore treatment of all AKs is recommended.3
Many treatments exist for AKs. Single or few discrete AKs are typically treated with cryosurgery.1 Treatment of multiple lesions and surrounding photo-damaged skin (field cancerization) includes topical agents and photodynamic therapy (Table 1).1 These treatments may be associated with local skin reactions of pain, irritation, redness, flaking, erosions, ulcerations, and irreversible skin changes of pigmentation and scarring.1 Furthermore, some treatments have to be administered over periods of weeks or months, which may reduce adherence and undermine treatment success.1
|Medication||Mechanism of Action||Dosing/ Application||Efficacy||Drawback/Adverse Effects||Contraindications|
|Tirbanibulin 1% ointment||Microtubule inhibitor and Src kinase inhibitor with potent antiproliferative activity against keratinocyte growth||Applied once daily on face or scalp for 5 consecutive days; up to 25 cm2 contiguous treatment surface||• 49% complete (100%) clearance of lesions • 72% partial reduction (>75%) of lesions • Median reduction in lesion count was 87.5%|| • Local skin reactions:
• Scaling, crusting, swelling, vesiculation/ pustulation and erosion/ ulceration observed less commonly
|5-fluorouracil 5% cream||Antimetabolite cytotoxic agent (antipyrimidine group); interferes with DNA synthesis||Applied to affected areas 1-2 times daily for 3-4 weeks||• 38% of patients experienced complete clearance of lesions in 6 months8 • Overall 73% reduction in lesions8||• Local skin reactions: pain, pruritus, burning, erythema, erosion, inflammation, hyperpigmentation||Pregnant women, patients with dihydropyrimidine dehydrogenase enzyme deficiency, and/or hypersensitivity to any components of the cream|
|Diclofenac 3% gel||Inhibits the cyclooxygenase pathway, resulting in decreases in prostaglandin E2 synthesis||Applied twice-daily applications for 8-12 weeks; up to 25 cm2 treatment surface||• 47% of patients experienced complete clearance at 90 days of twice daily application|| • Local skin reactions:
edema, pruritus, scaly
vesiculobullous rash |
• Long duration
• No systemic adverse events
|Patients with diclofenac, polyethylene glycol monomethyl ether 350, benzyl alcohol, and/or hyaluronate sodium hypersensitivity|
|Imiquimod 3.75% cream||Imidazoquinoline-derivative promotes activation of innate immunity||One cycle of 3 applications per week for 4 weeks, cycle can be repeated once; up to 25 cm2 contiguous treatment surface||• 34% of patients experienced complete clearance • 54% of patients experienced partial clearance|| • Local skin reactions:
erythema, pain, edema,
• Systemic reactions are rare
• Reactions occur less during a second treatment cycle
Table 1. Topical FDA-approved field therapies for AKs of the scalp and face.
Modified from: Dao D-PD, et al. 20214 and Dlott AH, et al. 202112
More recently, the role of the Src kinase in carcinogenesis has shed light on an alternative therapeutic option. In UV damaged skin, a cascade of events activates peroxisome proliferator activated receptor (PPAR) beta/delta which stimulates the Src oncogene expression, increases Src kinase activity and enhances the EGFR/Erk1/2 signaling pathway, resulting in increased epithelial-to-mesenchymal transition (EMT) marker expression.6 Elevated levels of Src have been linked to AKs and SCCs, and play a role in both primary tumor growth and metastases.7 Therefore, Src inhibitors were viewed as a plausible therapeutic option and many have since been developed. Tirbanibulin is a novel compound that inhibits Src kinase signaling and tubulin polymerization in rapidly dividing cells. It has shown promise as a new therapeutic agent for the treatment of AKs on the face or scalp.
Mechanism of Action
The chemical name of tirbanibulin is N-benzyl-2-(5-(4-(2-morpholinoethoxy)phenyl)pyridine-2-yal) acetamide (Figure 1).4 It is a synthetic, first-in-class, anti-proliferative agent that disrupts Src kinase signaling and inhibits tubulin polymerization (Figure 2).8 Through these mechanisms, it also promotes the induction of p53, G2/M arrest of proliferating cell populations, and subsequent apoptosis.8
The Phase I trial was an open-label, single-center study in subjects aged ≥18 years with clinically typical AKs on the forearm. Thirty participants were enrolled into 4 sequential cohorts (n=4, 10, 8, and 8 in each cohort, respectively): Cohort 1 received tirbanibulin ointment 1% 50 mg/day once daily for 3 days over 25 cm2 treatment area with 4-8 AK lesions; Cohort 2 received 200 mg/day once daily for 3 days over 100 cm2 treatment area with 8-16 AK lesions; Cohort 3 and Cohort 4 were similar to Cohort 1 and Cohort 2, respectively, but treatment was for 5 days. The follow-up period was through day 45.
To assess tirbanibulin activity, AK lesion numbers at baseline (day 1), days 10, 17, 31, and 45 were collected. Complete (100%) and partial (≥75%) AK clearance rates (defined by the reduction in AK lesions in the treatment area at day 45 compared with baseline) were evaluated for each cohort.
Twenty-nine participants completed the study with one participant withdrawing consent on day 2. Reductions in lesion counts from day 1 to 45 were observed in all cohorts. On day 45, Cohorts 1-4 demonstrated 25%, 0%, 50%, and 12.5% of complete AK clearance in the treatment area, respectively.
Dermal safety clinical studies in healthy subjects demonstrated that tirbanibulin 1% ointment did not cause contact sensitization, phototoxic skin reactions, or photoallergic skin reactions.4
The Phase II trial was an open-label, uncontrolled, dose-regimen- finding, multicenter study in subjects aged ≥18 years with clinically typical AKs on the face or scalp. One hundred and sixty-eight participants were enrolled into 2 sequential cohorts (n=84 in each cohort): Cohort 1 received 50 mg/day tirbanibulin ointment 1% once daily for 3 days over 25 cm2 treatment area with 4–8 AK lesions; Cohort 2 received the same treatment for 5 days.
AK lesion counts at baseline (day 1), 8, 15, 29, and 57 were collected, with a 12-month follow-up period for participants that achieved complete AK clearance to monitor for recurrence. All 168 participants completed the trial. Extensive overall AK clearance on the face or scalp was demonstrated in both cohorts. More participants had 100% clearance at day 57 in the 5-day (43% [95% Confidence Interval (CI) = 32, 54]) vs. the 3-day cohort (32% [95% CI = 22, 43]). Partial clearance rates were also slightly higher in the 5-day (56% [95% CI = 45, 67]) vs. the 3-day cohort (52% [95% CI = 41, 63]). There was a consistent decrease in lesion counts across all visits from baseline to day 57 for both cohorts.
All 63 participants who had 100% clearance at day 57 in the Phase 2 study were included in the Recurrence Follow-up Set. At 12 months post-day 57, recurrence rates for the 5-day cohort (57% [95% CI = 41, 73]) were lower than the 3-day cohort (70% [95% CI = 51, 87]). Most recurrence occurred within 6 months post-day 57.
The Phase III trial was a randomized, double-blind, parallel-group, vehicle-controlled, multicenter (62 US centers) trial in subjects aged >18 years with 4-8 clinically typical AKs on the face or scalp within a contiguous area measuring 25 cm2. A total of 702 participants, divided among two identical trials (n=351 at each site), were randomly assigned in a 1:1 ratio to receive either tirbanibulin 1% ointment for 5 days self-administered to a 25 cm2 contiguous area or vehicle ointment (placebo). Enrollment across patients was controlled to achieve a 2:1 ratio of facial: scalp treatment areas. The primary outcome was the percentage of patients with a complete reduction in the number of lesions in the application area at day 57. The secondary outcome was the percentage of patients with a partial reduction in the number of lesions within the application area at day 57. The incidence of recurrence was evaluated at 1 year.
Tirbanibulin demonstrated higher complete clearance levels in trial 1 (44% [95% CI = 32, 47]; P<0.001) and trial 2 (54% [95% CI = 33, 51]; P<0.001) compared to the vehicle groups (5% and 13% in trials 1 and 2, respectively). Pooled data from both trials demonstrated complete clearance in 49% ([95% CI = 35, 47]; P<0.001) of patients in the tirbanibulin groups and in 9% of the vehicle groups.
Partial clearance was significantly higher in both tirbanibulin groups at 68% (trial 1) and 76% (trial 2) than in the vehicle groups. Pooled data revealed partial clearance in 72% ([95% CI = 48, 60]; P<0.001) of patients in the tirbanibulin groups and in 18% of the vehicle groups. At 1 year, 47% of patients with complete clearance experienced recurrence of AKs.
Median reduction in AK lesion count in patients received tirbanibulin was 87.5% vs. 20% for vehicle (P<0.0001).1
Safety and Tolerability
Throughout all phases, no serious adverse events (AEs) were reported secondary to tirbanibulin.1,8 Local skin reactions (LSRs) consisted mostly of mild-moderate erythema, flaking or scaling, application-site pruritus, and application-site pain.1 Erythema (93%) and flaking (82%) were most common.1 Severe LSRs (all types) were observed in <10% of patients, with severe erythema in 6% of patients.1 Other LSRs included crusting, swelling, scaling, vesiculation or pustulation, and ulcerations or erosions.4 LSRs typically appeared on treatment day 2 and peaked on treatment day 8, before spontaneously resolving in ~2 weeks-1 month.1,8 No participants were withdrawn from the clinical trials due to AEs or LSRs.
Laboratory evaluation of blood chemistry, hematology, urine analysis, vital signs, electrocardiograms, and physical examinations in association with the phase III trials were not indicative of any systemic side effects.1 Tirbanibulin produces adverse ophthalmic reactions, and therefore, patients must be careful not to transfer the drug into the periocular area or eyes.
There has been no data on possible birth defects and/or adverse fetal/maternal outcomes during pregnancy with the use of tirbanibulin.4 However, extremely high doses were noted to cause birth defects in rats and rabbits (more than 70 and 159 times the recommended human dose, respectively).4
The topical 1% ointment formulation of tirbanibulin was approved by the US FDA in December 2020 for the treatment of AKs on the face and scalp.7 One packet of ointment (250 mg) contains 2.5 mg (1%) of tirabanibulin.4 The medication is applied on the affected area of the face or scalp once a day for 5 consecutive days.4 Each packet will cover up to 25 cm2 on the face or scalp and is disposed of after its one-time use.4
There are no contraindications listed in the FDA-approved prescribing information.4
Compared to other older topical treatments for AKs, tirbanibulin demonstrates several advantages. Tirbanibulin effectively and completely cleared AK lesions with a 5-day application while demonstrating a favorable safety profile and long-term results. The most common AEs for tirbanibulin were mild and included erythema, flaking, pruritus and pain at the application site. Unlike most other topical treatments for AKs, severe local
reactions, including vesiculation or pustulation and erosion or ulceration, were infrequent.1 The favorable tolerability is attributed to tirbanibulin mechanism of action in which it induces apoptosis rather than necrosis. Apoptosis is associated with little or no inflammation.10
Treatment adherence and patient satisfaction has shown to be significantly better with shorter duration topical treatments for AK.11 Current first-line topical field therapies for AKs include 5-fluorouracil (5-FU), imiquimod, and diclofenac gel, all of which have unfavorable aspects undermining compliance and thus successful treatment (Table 1).12 These therapies more frequently result in AEs, severe LSRs, and prolonged patient discomfort, at times necessitating a pause in treatment until the healthy skin has healed.12 In addition, each requires an extended period of application ranging from 3-4 weeks to 8-12 weeks with more than one application daily and/or a second cycle.12 Ingenol mebutate, a promising short duration (3-day) AK treatment, recently lost its first-line status after being discontinued in 2020 secondary to increased risk of skin malignancy.
A combination of 5% 5-FU cream plus 0.005% calcipotriol ointment has been used off-label to treat AKs with variable results.13 The evidence was based on a single center study using the combination topical treatment twice daily for 4 days applied to 25 cm2 area.13 Twenty-seven percent of patients achieved complete clearance of AK lesions on the face, and <20% on the scalp at week 8.13 Severe erythema on the face was observed in 80% of patients.13
The incidence of recurrence with conventional treatments has ranged from 20% to 96%.1 However, owing to the chronic nature of AKs, recurrence of lesions in sun-damaged areas is expected.1 With tirbanibulin, 47% of patients with complete clearance experienced a recurrence at 1 year in the phase III trials. Despite the apparent benefits of tirbanibulin, no direct comparisons have been made with other AK treatments.1 Ultimately, head-to-head studies will be needed to determine true superiority/inferiority.
Other uses of tirbanibulin are being explored. Phase I trials are underway in Taiwan to investigate its potential as a treatment for psoriasis.7 Tirbanibulin was noted to successfully eradicate periungual SCC in a patient that failed to clear with imiquimod 5% in combination with monthly cryotherapy, suggesting its potential as a nonsurgical therapeutic option for SCC.14
Early and effective treatment of AKs is important to prevent progression to SCC.4 Many topical treatments for AKs are often limited because of patient tolerability, treatment duration, and adherence. Tirbanibulin effectively and completely cleared AK lesions with a 5-day application period while demonstrating a favorable safety profile, mild AEs, improved tolerability, and long-term results, making it a promising field therapy for AKs.
- Blauvelt A, Kempers S, Lain E, et al. Phase 3 Trials of tirbanibulin ointment for actinic keratosis. N Engl J Med. 2021 Feb 11;384(6):512-20.
- Reinehr CPH, Bakos RM. Actinic keratoses: review of clinical, dermoscopic, and therapeutic aspects. An Bras Dermatol. 2019 Dec;94(6):637-57.
- Dianzani C, Conforti C, Giuffrida R, et al. Current therapies for actinic keratosis. Int J Dermatol. 2020 Jun;59(6):677-84.
- Dao DD, Sahni VN, Sahni DR, et al. 1% tirbanibulin ointment for the treatment of actinic keratoses. Ann Pharmacother. 2022 Apr;56(4):494-500.
- Balcere A, Rone Kupfere M, Čēma I, et al. Prevalence, discontinuation rate, and risk factors for severe local site reactions with topical field treatment options for actinic keratosis of the face and scalp. Medicina (Kaunas). 2019 Apr 4;55(4):E92.
- Montagner A, Delgado MB, Tallichet-Blanc C, et al. Src is activated by the nuclear receptor peroxisome proliferator-activated receptor β/δ in ultraviolet radiation-induced skin cancer. EMBO Mol Med. 2014 Jan;6(1):80-98.
- Markham A, Duggan S. Tirbanibulin: first approval. Drugs. 2021 Mar;81(4): 509-13.
- Kempers S, DuBois J, Forman S, et al. Tirbanibulin ointment 1% as a novel treatment for actinic keratosis: phase 1 and 2 results. J Drugs Dermatol. 2020 Nov 1;19(11):1093-100.
- Bartlett G. Line diagram of tirbanibulin [Internet]. 2020 Dec 17 [cited 2021 Oct 20]. Available from: https://commons.wikimedia.org/wiki/File:Tirbanibulinline.png.
- Wallach D, Kovalenko A. Keeping inflammation at bay. Elife. 2014 Mar 25;3:e02583.
- Grada A, Feldman SR, Bragazzi NL, et al. Patient-reported outcomes of topical therapies in actinic keratosis: a systematic review. Dermatol Ther. 2021 Mar;34(2):e14833.
- Dlott AH, Di Pasqua AJ, Spencer SA. Tirbanibulin: topical treatment for actinic keratosis. Clin Drug Investig. 2021 Sep;41(9):751-5.
- Cunningham TJ, Tabacchi M, Eliane J-P, et al. Randomized trial of calcipotriol combined with 5-fluorouracil for skin cancer precursor immunotherapy. J Clin Invest. 2017 Jan 3;127(1):106-16.
- Moore AY, Moore S. Topical tirbanibulin eradication of periungual squamous cell carcinoma. JAAD Case Rep. 2021 Jun 26;14:101-3.